| 货号 | 39160 |
| 同种亚型 | Serum |
| 反应种属 | Budding Yeast, Human, Mouse, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Immunofluorescence/Dot Blot/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 3 - 5 µl per ChIP ChIP-Seq: 3 µl each ICC/IF: 1:500 - 1:1,000 dilution WB: 1:1,000 - 1:5,000 dilution modENCODE validation. This antibody was validated for ChIP-Seq in this study (see reference). NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here.. Published Applications: ChIP,ChIP-chip,&ChIP-Seq See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif | |
| 纯化方式 | None | |
| 免疫原 | This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3. | |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4. | |
| 运输条件 | Blue Ice | |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. | |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an 计算分子量 | 17 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Histone H3 trimethyl Lys4 antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2 million HL-60 cells and 3 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 19 million sequence tags were mapped to identify H3K4me3 binding sites. The image shows H3K4me3 bound to most promoters of genes in the ZNF gene cluster on chromosome 19. (Gene annotations run from right to left, therefore TSSs are on the right side of each gene annotation.) | |
Histone H3 trimethyl Lys4 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 2 million HL-60 cells and 3 µl of Histone H3 trimethyl Lys4 antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction. | |
Histone H3 trimethyl Lys4 antibody tested by immunofluorescence. HeLa cells stained at 1:1,000 with Histone H3 trimethyl Lys4 antibody. Top left: Histone H3 trimethyl Lys4 antibody.Top right: Stained with DAPI. Bottom left: Same cells stained with alpha Tubulin mAb (Clone 5-B-1-2). Bottom right: Merge of all 3 images. | |
Histone H3 trimethyl Lys4 antibody tested by Western blot. HeLa nuclear extract (20 µg) was probed with Histone H3 trimethyl Lys4 antibody (1:2,000 dilution). | |
Histone H3 trimethyl Lys4 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 trimethyl Lys4 antibody for trimethyl-lysine 4 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation (lysine 4, lysine 9, lysine 27) were spotted onto PVDF and probed with the antibody at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified H3K4. Lane 2: H3K4me1. Lane 3: H3K4me2. Lane 4: H3K4me3. Lane 5: unmodified H3K9. Lane 6: H3K9me1. Lane 7: H3K9me2. Lane 8: H3K9me3. Lane 9: unmodified H3K79. Lane 10: H3K79me1. Lane 11: H3K79me2. Lane 12: H3K79me3. Bottom panel - Lane 1: unmodified H3K23. Lane 2: H3K23me1. Lane 3: H3K23me3. Lane 4: H3K23me3. Lane 5: unmodified H3K27. Lane 6: H3K27me1. Lane 7: H3K27me2. Lane 8: H3K27me3. Lane 9: unmodified H3K36. Lane 10: H3K36me1. Lane 11: H3K36me2. Lane 12: H3K36me3. |
