| 货号 | 39114 |
| 同种亚型 | Serum |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Rabbit |
| 应用 | ChIP/ChIP-Seq/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 2 - 10 µl per ChIP ChIP-Seq: 5 µl each WB: 1:1,000 - 1:5,000 dilution Published Applications: ChIP ChIP-Seq WB See references for more information. Individual optimization may be required. This histone variant can be perturbed by excessive washing steps prior to fixation. Cells should be washed into serum-free culture medium (NOT PBS!) prior to fixation. |
| 供应商 | Active Motif |
| 纯化方式 | None |
| 免疫原 | This Histone H2A.Z antibody was raised against a peptide derived from the C-terminus of human histone H2A.Z. |
| 背景 | Thenucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H2A.Z (H2AZ, H2AFZ) is a histone H2A variant, a protein similar to canonical H2A but with different molecular identity and unique functions. H2A.Z is highly conserved during evolution. It plays an important role in basic cellular mechanisms such as gene activation, chromosome segregation, heterochromatic silencing and progression through the cell cycle. H2A.Z is acetylated at multiple lysine residues in its amino terminus, which may serve to allow H2A.Z to function as an insulator of chromatin domains. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an Ig |
| 计算分子量 | 14 kDa |
| 参考文献 |
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Histone H2A.Z antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the human H9 embryonic stem cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify H2A.Z occupancy. H2A.Z is found throughout the genome, is often enriched at promoters and depleted from transcribed genes as shown in the images. | |
Histone H2A.Z antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2A.Z pAb or 10 µl of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method. | |
Histone H2A.Z antibody tested by Western blot. Detection of H2A.Z by Western blot. The analysis was performed using Histone H2A.Z pAb at a 1:5,000 dilution. Lane 1: 20 ng recombinant histone H2A.Z protein. Lane 2: 5 µg HeLa acid extract. Lane 3: 5 µg HeLa acid extract plus 1 µM immunizing peptide. | |
Histone H2A.Z antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H2A.Z antibody for histone H2A.Z. Recombinant proteins corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:4,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: recombinant Histone H2A. Lane 2: recombinant Histone H2A.Z. Lane 3: recombinant Histone H4. |
