| 货号 | 40600 |
| 使用方法 | COS-7 nuclear extract (CoCl2 treated) is specifically recommended for 1) analysis of replication, 2) for transfection studies and 3) for studies related to hypoxia. |
| 供应商 | Active Motif |
| 背景 | COS-7 nuclear extract (CoCl2treated) was prepared from subconfluent undifferentiated cell cultures of the Simian kidney fibroblast COS-7 cell line. The COS-7 cell line was derived from immortalizing the African green monkey CV-1 cell line by transformation with an origin-defective mutant of the SV40 viral genome that is replication-deficient but produces wild-type large T antigen. The large T antigen supports replication of and lytic growth of the SV40 virus as well as replication of SV40 promoter-containing plasmids. In addition to supporting propagation of the Simian SV40 virus, the COS-7 cell line is most commonly used in research related to replication and in transfection studies for the production of recombinant proteins. Treatment of COS-7 cells with CoCl2 induces hypoxia. Hypoxia is a significant contributor to various developmental and pathophysiological processes. These include cell differentiation, wound healing, angiogenesis and various human diseases, including ischemia, cancer, heart disease pulmonary disease, and inflammation. Hypoxic conditions induce an increase in HIF-1, a transcription factor involved in mammalian oxygen homeostasis. This leads to upregulation of transcription of genes that promote cell survival in low-oxygen conditions, mainly the PI3K/Akt pathway that is important for cell survival. In addition, hypoxia induces proliferation and differentiation of various differentiating cell types, particularly those related to the neural system and vasculogenesis. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C. We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 2 x 100 µg of COS-7 nuclear extract (CoCl2 treated) at 2.5 µg/µl. |
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Figure 1: Measurement of HIF-1α. Nuclear extracts were isolated from COS-7 cells using the Nuclear Extract Kit. The cells were either treated for 20 hours with 0.15 mM CoCl2 or were untreated. Increasing amounts of nuclear extract are assayed for HIF-1α activation using the TransAM HIF-1 Kit. |
