K-562 nuclear extract
货号：
36015 基本售价：
2465.0 元 规格：
200 ug
产品信息
概述| 货号 | 36015 |
| 使用方法 | K-562 nuclear extract is recommended for use in the studies related to 1) differentiation, 2) immunology, and 3) cancer. |
性能| 供应商 | Active Motif |
| 背景 | K-562 nuclear extract was prepared from a cell culture of the K-562 human myelogenous leukemia cell line. The K-562 cell line was originally derived from immortalized erythroleukemia cells obtained from a patient with a Philadelphia chromosome-positive chronic myeloid leukemia in blast crisis. These pluripotent cells are widely applicable in research due to their ability to spontaneously differentiate into erythrocyte, granulocyte and monocyte progenitors. K-562 cells are also frequently utilized in in vitro natural killer (NK) cell assays as highly sensitive targets since they lack the expression of MHC class I molecules required for protection against NK activity. |
| 运输条件 | Dry Ice |
| 存放说明 | To ensure stability, extracts should be stored at -80°C.
We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles. |
| 外观 | 200 µg of K-562 nuclear extract at 4.3 µg/µl. |
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参考图片Figure 1: Monitoring GATA family member activation using the TransAM GATA Family Kit.
GATA-1, -2 and -3 activation were assayed using the TransAM GATA Family Kit. 1:1000 dilutions of each antibody in the kit were tested using 5 µg/well of nuclear extract prepared from an unstimulated cell line: GATA-1 and -2 were tested with K-562 and GATA-3 with Jurkat. Assays were performed in the absence or presence of 20 pmol of competitor oligonucleotide that contains either a wild-type or mutated GATA consensus binding site. Note that the wild-type oligonucleotide reduces GATA binding by over 90%, while incubation with the mutant GATA competitor oligo has a limited effect on GATA-1, -2 and -3 binding to DNA. |
