Quick ChIP Kit
货号：
NBP2-29902 基本售价：
4758.9 元 规格：
1 Kit
产品信息
概述| 货号 | NBP2-29902 |
| 反应种属 | Hu, Mu, BaSpecies Glossary |
| 应用 | ChIP, IP |
性能| 供应商 | Novus |
| 背景 | Chromatin Immunoprecipitation (ChIP) is a powerful method used to identify regions of the genome associated with specific proteins. These associations are crucial for vital cellular functions including gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing.1 The ChIP assay has been used to study both histones and non-histone proteins, such as transcription factors, within the context of the cell.2,3,4,5 Transcription factors and other DNA binding proteins have a weaker affinity than histones, which generally are tightly associated within the chromatin complex. To avoid dissociation of non-histone proteins from the chromatin binding site, it is necessary to incorporate a cross-link step. QuikChIPTM Kit has been optimized for use with both histone and non-histone proteins. Using the QuikChIPTM Assay, cells are fixed with formaldehyde to cross-link nonhistone proteins to DNA. Alternatively, the cross-linking steps are not needed for histone proteins, which are tightly bound to DNA. The chromatin are released from the nuclei and sheared into 200-1000 bp fragments. The chromatin are then immunoprecipitated with specific antibodies. DNA sequences cross-linked to the protein of interest co-precipitate as part of the chromatin complex. The DNA/Chromatin/Antibody complex is then isolated using Protein G Agarose. After reverse cross-linking, the associated DNA is released from the complex and is ready for analysis. |
| 存放说明 | Storage is content dependent. |
参考图片Chromatin Immunoprecipitation: QuickChip (with control) ChIP Kit [NBP2-29902] - Human MCF-7 cells were fixed for 10 minutes with 1% formaldehyde. The cells were lysed and the chromatin sonicated (200-1000 bp fragments). Chromatin was immunoprecipitated using DNMT3b mAb, (Lane 2) and a no antibody control (Lane 3). The PCR amplification was performed, with primers specific to the progesterone receptor (PR) promoter region, as follows: 94C 5 min, 94C 30 s, 59C 30 s 40 cycles, 72C 30 s, 72C 10 min. Following PCR, 20 ul of each sample was analyzed on a 2% agarose gel and visualized by UV-illumination following ethidium bromide staining. PCR product was observed in the DNMT3b mAb ChIP (Lane 2) and not in the control (Lane 3). |
