Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
Sci Rep, 2016;6(0):32470.
Species: Human
Sample Type: Whole Cells
Application: Flow
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
J Invest Dermatol, 2015;135(5):1311-9.
Species: Human
Sample Type: Whole Cells
Application: FACS sorting
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
BMC Cancer, 2015;15(0):351.
Species: Human
Sample Type: Whole Cells
Application: Flow
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
J Immunol, 2015;195(2):736-48.
Species: Human
Sample Type: Whole Cells
Application: Neut
MicroRNA editing facilitates immune elimination of HCMV infected cells.
Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
PLoS Pathog, 2014;10(2):e1003963.
Species: Human
Sample Type: Whole Cells
Application: Flow
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
Authors: Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prodhomme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P
PLoS Pathog, 2014;10(5):e1004058.
Species: Human
Sample Type: Whole Cells
Application: Neut
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
Authors: Esteso G, Luzon E, Sarmiento E, Gomez-Caro R, Steinle A, Murphy G, Carbone J, Vales-Gomez M, Reyburn H
J Immunol, 2014;193(3):1344-52.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
PLoS ONE, 2012;7(2):e31959.
Species: Human
Sample Type: Whole Cells
Application: Flow
MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer Res, 2012;72(21):5463-72.
Species: Human
Sample Type: Whole Cells
Application: Flow
DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
Authors: Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A
Blood, 2011;117(18):4778-86.
Species: Human
Sample Type: Whole Cells
Application: Flow
Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
PLoS ONE, 2011;6(2):e16899.
Species: Human
Sample Type: Cell Lysates
Application: WB
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
J. Exp. Med., 2011;208(1):13-21.
Species: Human
Sample Type: Whole Cells
Application: Flow
Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
Authors: Ohashi M, Eagle RA, Trowsdale J
J. Biol. Chem., 2010;285(22):16408-15.
Species: Human
Sample Type: Whole Cells
Application: Flow
Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
Blood, 2010;115(25):5170-9.
Species: Human
Sample Type: Whole Cells
Application: Flow
Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation.
Authors: Wu JD, Atteridge CL, Wang X, Seya T, Plymate SR
Clin. Cancer Res., 2009;15(2):632-40.
Species: Mouse
Sample Type: Whole Cells
Application: Flow
Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
Blood, 2009;113(13):2955-64.
Species: Human
Sample Type: Whole Cells
Application: Neut
Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein.
Authors: Boutet P, Aguera-Gonzalez S, Atkinson S, Pennington CJ, Edwards DR, Murphy G, Reyburn HT, Vales-Gomez M
J. Immunol., 2009;182(1):49-53.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Mol. Immunol., 2008;45(15):3917-25.
Species: Human
Sample Type: Whole Cells
Application: Flow
Induction of MHC class I-related chain B (MICB) by 5-aza-2-deoxycytidine.
Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
Biochem. Biophys. Res. Commun., 2008;370(4):578-83.
Species: Human
Sample Type: Whole Cells
Application: Flow
Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
Cancer Res., 2005;65(23):11136-45.
Species: Human
Sample Type: Whole Cells
Application: Flow

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Salih, H. et al. (2003) Blood 102:1389.