| 货号 | 7831S |
| 描述 | CSTs PathScan® Total IκBα Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan®Total IκBα Sandwich ELISA Kit #7360. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IκBα Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by an IκBα Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total IκBα protein. *Antibodies in this kit are custom formulations specific to the kit. |
| 反应种属 | Human/Mouse |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). |
| 存放说明 | 4C |
| 参考文献 | Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63. |
The relationship between lysate protein concentration from untreated and TNF-α and IL-1β treated HeLa cells and the absorbance at 450 nm using PathScan® Total IκBα Sandwich ELISA Antibody Pair #7831 is shown. HeLa cells were treated with TNF-α and IL-1β for 5 minutes at 37ºC and then lysed.未经处理和经TNF-α 和 IL-1β 处理的HeLa细胞的裂解液蛋白浓度与450 nm 吸收峰的关系，所用抗体为PathScan® Total IκBα Sandwich ELISA Antibody Pair #7831 。在37ºC条件下，用TNF-α和IL-1β 处理HeLa细胞5min，然后裂解。 |
