| 货号 | 7217S |
| 描述 | CSTs PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Kit #7325. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The SAPK/JNK Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Phospho-SAPK/JNK (Thr183/Tyr185) Detection Antibody and Anti-rabbit IgG, HRP-conjugated Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-SAPK/JNK (Thr183/Tyr185) protein. *Antibodies in this kit are custom formulations specific to the kit. |
| 反应种属 | Human/Mouse |
| 应用 | ELISA |
| 目标/特异性 | For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5). |
| 存放说明 | 4C |
| 参考文献 | Davis, R.J. (1999) Biochem Soc Symp 64, 1-12. Ichijo, H. (1999) Oncogene 18, 6087-93. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5. |
The relationship between lysate protein concentration from untreated and UV-treated 293 cells and the absorbance at 450 nm using PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair #7217. 293 cells were treated with UV, allowed to recover for 30 minutes at 37ºC and then lysed.未经处理的和用紫外照射的293细胞裂解物中蛋白质浓度之间的关系和450 nm处的吸光率已表示出来。使用的抗体是PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair #7217。293细胞先用紫外照射37ºC恢复30 min后，再裂解。 |
