Recombinant NFκB p65 protein
货号：
31302 基本售价：
12542.5 元 规格：
15 ug
产品信息
概述| 货号 | 31302 |
| 使用方法 | Recombinant NFκB p65 is suitable for Western blotting and TransAM® assays. 100 ng is sufficient for most protein-protein interaction studies. The standard curve for TransAM NFκB p65 was generated using the range of 40-0.625 ng of protein.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. |
性能| 供应商 | Active Motif |
| 背景 | NFκB p65 is a subunit of the NFκB transcription factor complex that plays a significant role in the regulation of genes that control various biological processes, including inflammation, differentiation, tumorigenesis, and cell growth and survival. NFkB is comprised of homo- or heterodimers of different subunits of the structurally related Rel family of transcription factors that includes p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), RelB and c-Rel.NFκB p65, RelB and c-Rel contain a transactivation domain (TD) in their C-termini, which is required for the transport of active NFkB complexes into the nucleus. In contrast, subunits p50 and p52 do not contain transactivation domains; they are unable to transactivate on their own and must form heterodimers with p65, RelB or c-Rel. The p50/p65 heterodimers and the p50 homodimers are the most common dimers found in the NFκB signaling pathway. Inactive NFkB dimers are sequestered in the cytoplasm of cells by the IκB family of inhibitory proteins. Activation of NFkB by external inducers such as lipopolysaccharide, TNF or IL-1, results in the phosphorylation and degradation of the IκB proteins. This releases NFκB dimers, which subsequently translocate to the nucleus where they activate appropriate target genes. |
| 运输条件 | Dry Ice |
| 存放说明 | Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 5 µg of Recombinant NFκB p65 protein 1X Protein Dilution Buffer (20 mM Tris-HCl, 0.2 M NaCl, 2 mM MgCl2 and 10% glycerol) and 1 M DTT. Protein is supplied at 100 ng/µl in Protein Dilution Buffer. |
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参考图片TransAM® standard curve generated using Recombinant NFκB p65 protein.
The standard curve for TransAM® was generated using a range of 40 - 0.625 ng of protein and run on the TransAM® NFκB p65 ELISA Kit. |
