Recombinant p53 protein
货号：
31103 基本售价：
6742.5 元 规格：
5 ug
产品信息
概述| 货号 | 31103 |
| 别名 | TP53, BCC7, LFS1, TRP53 |
| 使用方法 | Recombinant p53 is suitable for TransAM®, protein-protein and DNA protein assays. 1 ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40 – 0.625 ng of protein.
NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided. |
性能| 供应商 | Active Motif |
| 背景 | p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFκB (NFκB p50 and NFκB p65), both of which serve to inhibit apoptosis. |
| 运输条件 | Dry Ice |
| 存放说明 | Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 5 µg of Recombinant p53 protein and 1X Protein Dilution Buffer (20 mM Tris-HCl pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA and 1 mM DTT. Protein is supplied at 0.5 µg/µl in Protein Dilution Buffer. |
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参考图片TransAM® standard curve generated using Recombinant p53 protein.
The standard curve for TransAM® was generated using a range of 40 – 0.625 ng of protein and run on the TransAM® p53 ELISA Kit. |
