| 货号 | 31452 |
| 别名 | SET Domain Bifurcated 1, ERG-Associated Protein With SET Domain, Histone H3-K9 Methyltransferase 4 |
| 使用方法 | Recombinant SETDB1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: H3K9me3 methyltransferase. HMT Assay Conditions: 30 ng/μl calf thymus Histone octamers were incubated with 30 ng/μl recombinant SETDB1 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | SETDB1 (SET Domain, Bifurcated 1) is a histone methyltransferase that methylates histone H3 at lysine 9. H3K9 methylation is associated with transcriptional repression and gene silencing through recruitment of HP1 proteins to methylated histones. H3K9 trimethylation is coordinated with DNA methylation, most likely through formation of a complex with MBD1 and ATF7IP that leads to transcriptional repression. SETDB1 activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28, a factor recruited by KRAB zinc-finger proteins. Aberrant SETDB1 function is associated with the pathogenesis of Huntington Disease. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant SETDB1 protein expressed in Sf9 cells at a concentration of 0.6 mg/ml in 25 mM HEPES, pH 7.5, 120 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. |
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Recombinant SETDB1 protein gel. SETDB1 protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
SETDB1 activity assay. HMT assay measuring the activity of Recombinant SETDB1. Activity was detected by fluorography. |
