| 货号 | 31423 |
| 使用方法 | Recombinant MLL1 complex is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4 methyltransferase Catalytic Ability: ~300 turnovers/ enzyme molecule HMT Assay Conditions: 3.3 μM H3K4me0 (1-21aa) peptide was incubated with different concentration of recombinant MLL1 Complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF. |
| 供应商 | Active Motif |
| 背景 | MLL1 (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog 1, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of ‘Lys-4’ of histone H3 (H3K4me) complex and acetylation of ‘Lys-16’ of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on Thr-3, less activity toward H3 dimethylated on Arg-8 or Lys-9, while it has higher activity toward H3 acetylated on Lys-9. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant MLL1 Complex is supplied at a concentration of 0.75 µg/µl in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol and 0.04% Triton X-100. |
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Recombinant MLL1 complex gel. Recombinant MLL1 complex was run on an 12% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant MLL1 complex activity assay. 3.3 uM H3K4me0 peptide was incubated with 10 nM MLL1 Complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. | |
Recombinant MLL1 complex activity assay. 3.3 μM H3K4me0 (1-21aa) peptide was incubated with MLL1 Complex in reaction buffer for 3 hour at room temperature. MLL1 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody. |
