| 货号 | 31399 |
| 别名 | HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP |
| 使用方法 | Recombinant SETD2 (1392-2564) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: H3K36 methyltransferase. HMT Assay Conditions: 30 ng/μl recombinant nucleosomes were incubated with 30 ng/μl recombinant SETD2 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II.Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 20 µg of recombinant SETD2 (1392-2564) protein expressed in Sf9 cells at a concentration of 0.5 mg/ml in 25 mM HEPES, pH 7.5, 120 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant SETD2 (1392-2564) protein gel. SETD2 (1392-2564) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. |
