| 货号 | 31394 |
| 别名 | HRMT1L6 |
| 使用方法 | Recombinant PRMT6 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: Specifically mediates the asymmetric dimethylation of histone H3 Arg-2 to form H3R2me2a. Also methylates histone H2A and H4 Arg-3 (H2AR3me and H4R3me, respectively). HMT Assay Conditions: 60 ng/ul Calf Thymus Octamers were incubated with 30 ng/ul recombinant PRMT6 protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 3 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | PRMT6 (Protein Arginine Methyltransferase 6, HRMT1L6, ANM6) is a type I arginine methyltransferase. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT6 preferentially methylates arginine residues present in GAR (glycine and arginine-rich) domains. PRMT6 asymmetrically dimethylates histone H3 at Arg2, which is mutually exclusive to methylation at lysine 4 (associated with transcriptional activation), and thus PRMT6 serves as a transcriptional repressor in this context. It can also symmetrically dimethylate H2A and H4 at Arg3 and serves as a coactivator of transcription in concert with nuclear receptors. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant PRMT6 protein was expressed in Sf9 cells at a concentration of 0.3 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
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PRMT6 activity assay. 30 ng/µl Recombinant Histone H3.1 (Cat# 31294) or 60 ng/µl Calf Thymus Octamer were incubated with 15 ng/µl PRMT6 (+) and 30 ng/µl PRMT6(++), respectively, in reaction buffer for 3 hours at room temperature. Activity was detected by fluorography. |
