| 货号 | 31388 |
| 使用方法 | PRC2 EZH2(Y641F) Complex is suitable for use in the study of enzyme kinetics, inhibitor screening and selectivity profiling.Specific Activity: H3K27me3 and H3K27me2 methyltransferase. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 3.3 μM H3K27me2, 40 nM Recombinant PRC2 EZH2(Y641F) Complex, 100 μM SAM at 2 hours at room temperature. Activity was detected by MALDI-TOF. |
| 供应商 | Active Motif |
| 背景 | PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at Lys27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of Lys27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation.Recombinant PRC2 EZH2(Y641F) Complex is a mutant version of our wild-type PRC2 Complex that contains a Tyr-to-Phe mutation at tyrosine 641 of the SET domain of EZH2. Somatic mutations of tyrosine 641, including Y641F, Y641N, Y641C, Y641S and Y641H, have been identified in patients with non-Hodgkin lymphoma and have been shown to alter substrate specificity and catalytic activity of EZH2 for histone H3 lysine 27 (H3K27) methylation states resulting in increased H3K27 trimethylation. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant PRC2 EZH2(Y641F) Complex expressed in Sf9 at a concentration of 0.7 mg/ml in 25 mM HEPES pH 7.5, 120 mM NaCl, 5% glycerol and 0.2 mg/ml 3X FLAG peptide. |
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Recombinant PRC2 EZH2(Y641F) Complex gel. PRC2 EZH2(Y641F) Complex was run on an SDS-PAGE gel and stained with Coomassie blue. | |
PRC2 EZH2(Y641F) Complex activity assay. Recombinant PRC2 EZH2(Y641F) Complex activity measured using a demethylation assay. MALDI-TOF was used for detection. |
