Recombinant JHDM1D-s protein
货号：
31463 基本售价：
6162.5 元 规格：
20 ug
产品信息
概述| 货号 | 31463 |
| 别名 | KDM7A, Lysine Demethylase 7A, Jumonji C Domain Containing Histone Demethylase 1 Homolog D |
| 使用方法 | Recombinant JHDM1D-s is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
性能| 供应商 | Active Motif |
| 背景 | JHDM1D(Jumonji C Domain Containing Histone Demethylase 1 Homolog D), also known as KIAA1718andKDM7, is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JHDM1D harbors two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression), Histone H3 dimethyl Lys36 (H3K36me2) and also Histone H4 monomethyl Lys20 (H4K20me1) via an oxidative pathway that requires the presence of Fe(II) and α-ketoglutarate as cofactors. In the presence of H3K4me3, JHDM1D has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. JHDM1D demethylates H3K9me2 in the absence of H3K4me3. JHDM1D has activity toward H4K20me1 only when a nucleosome is used as a substrate, not when a histone octamer is used as a substrate. JHDM1D enzyme is required for brain development. JHDM1D-s is a truncated protein (aa 92-488) that includes the enzymatic Jumonji domain of JHDM1D. The truncated protein behaves as a promiscuous enzyme, losing substrate specificity when the PHD domain is deleted. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 20 µg of recombinant JHDM1D-s protein expressed in E. coli cells and is supplied in 20 mM Bis-Tris, pH 5.5, 200 mM NaCl, 5% (v/v) glycerol and 1 mM DTT. Please refer to product insert upon arrival for lot-specific concentration. |
| 参考文献 | This protein is described in the following reference:
Horton J et al.(2010)Nature17:38-43
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