| 货号 | 31462 |
| 别名 | JHDM1F; MRXSSD; ZNF422 |
| 使用方法 | Recombinant PHF8-l is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | PHF8(PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B(KDM7B) and JmjC domain-containing histone demethylation protein 1D-B(JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1), Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2) via an oxidative pathway that requires the presence of Fe(II) and α-ketoglutarate as cofactors. Horton et al. (2010) demonstrated that, in the presence of H3K4me3, PHF8 shows enhanced demethylation of H3K9me2 on the same peptide. PHF8 enzyme is involved in various cellular processes, including DNA replication and repair and transcriptional regulation. Defects in the Jumonji domain of PHF8 lead to Siderius type X-linked mental retardation (MRXSSD).PHF8-l is a truncated protein (aa 1-447) that includes the N-terminal domain, including the PHD and the Jumonji catalytic domain of PHF8. The protein contains different segments for both recognizing and removing opposing methyl groups. While the PHD domain binds H3K4me3, an alteration associated with transcriptional activation, the Jumonji (catalytic) domain demethylates H3K9me2, a mechanism associated with transcriptional repression. The enzymes’ catalytic activity and substrate specificity is thereby enhanced through the simultaneous binding of multiple domains. The ability to distinguish the function of individual methyl marks could lead to a marked understanding of the language of the histone code. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 20 µg of recombinant PHF8-l protein expressed in E. coli cells at a concentration of 0.3 mg/ml in 20 mM Bis-Tris, pH 5.5, 200 mM NaCl, 5% (v/v) glycerol and 1 mM DTT. |
| 参考文献 | This protein is described in the following reference: Horton J et al.(2010)Nature17:38-43 |
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PHF8-l protein gel. PHF8-l (10 μg) run on a 4-20% SDS-PAGE gel and stained with Coomassie blue. | |
PHF8-l activity assay. Recombinant PHF8-l activity measured using a fluorescence-based demethylation assay. |
