| 货号 | 31500 |
| 别名 | Enhancer Of Zeste 1, EC 2.1.1.43 |
| 使用方法 | Recombinant EZH1 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K27me(1-3) methytransferase HMT Assay Conditions: 3.3 μM H3K27me0 (16-37aa) peptide was incubated with different concentrations of recombinant EZH1 Complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF. |
| 供应商 | Active Motif |
| 背景 | EZH1 (Enhancer Of Zeste Homolog 1 ) is a Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates ‘Lys-27’ of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate ‘Lys-27’ of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant EZH1 Complex was expressed in Sf9 cells and supplied at a concentration of 1 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
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Recombinant EZH1 Complex gel EZH1 Complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant EZH1 Complex HTRF activity assay 3.3 μM H3K27me0 (16-37aa) peptide was incubated with EZH1 Complex in reaction buffer for 3 hour at room temperature. EZH1 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K27me1) was measured using H3K27me1-specific antibody. |
