| 货号 | 31499 |
| 使用方法 | Recombinant MLL4 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me(1-2) methytransferase HMT Assay Conditions: 3.3 μM H3K4me0 (1-21aa) peptide was incubated with different concentrations of recombinant MLL4 Complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF. |
| 供应商 | Active Motif |
| 背景 | MLL4 (Myeloid/lymphoid or mixed-lineage leukemia 4) is a Trithorax-group protein that function collectively to promote gene expression. MLL4 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL4 plays a central role in β-globin locus transcription regulation by being recruited by Nfe2. MLL4 is also required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that precedes resumption of meiosis, oocyte survival and normal zygotic genome activation. MLL4 may also be involved in human cancer as its expression has been shown to be upregulated in solid tumor cell lines. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant MLL4 Complex was expressed in E.coli cells and supplied at a concentration of 1.4 μg/μl in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100. |
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Recombinant MLL4 Complex gel MLL4 Complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant MLL4 Complex HTRF activity assay 3.3 μM H3K4me0 (1-21aa) peptide was incubated with MLL4 Complex in reaction buffer for 3 hour at room temperature. MLL4 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody. | |
Recombinant MLL4 Complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 20 nM MLL4 Complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: 50 turnovers/ enzyme molecule |
