| 货号 | 31498 |
| 使用方法 | Recombinant MLL2 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me(1-3) methytransferase HMT Assay Conditions: 3.3 μM H3K4me0 (1-21aa) peptide was incubated with different concentrations of recombinant MLL2 Complex in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 3 hours at room temperature. Activity was detected by HTRF and MALDI-TOF. |
| 供应商 | Active Motif |
| 背景 | MLL2 (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. MLL2 is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. MLL2 is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant MLL2 Complex was expressed in E.coli cells and supplied at a concentration of 0.9 μg/μl in 25 mM Tris pH 8.0, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100. |
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Recombinant MLL2 Complex gel MLL2 Complex was run on an 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant MLL2 Complex HTRF activity assay 3.3 μM H3K4me0 (1-21aa) peptide was incubated with MLL2 Complex in reaction buffer for 3 hour at room temperature. MLL2 Complex was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me2) was measured using H3K4me2-specific antibody. | |
Recombinant MLL2 Complex MALDI-TOF activity assay 3.3 uM H3K4me0 peptide was incubated with 10 nM MLL2 Complex in reaction buffer for 3 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me0 peptide was used as negative control. Catalytic Ability: ~450 turnovers/ enzyme molecule |
