| 货号 | 31488 |
| 别名 | PRO9856, LAVS3040 |
| 使用方法 | Recombinant BRD9 (130-259) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | BRD9 (Bromodomain-containing protein 9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant BRD9 (130-259), GST-tag was expressed in E. coli cells at a concentration of 1.6 μg/μl in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant BRD9 (130-259) SDS-PAGE gel. BRD9 (130-259) protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant BRD9 (130-259) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD9 (130-259), GST-tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products |
