| 货号 | 31506-AM |
| 别名 | HD6, JM21, CPBHM, PPP1R90 |
| 使用方法 | Recombinant HDAC6 (597-728, deleted mutant) protein is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. HDAC Activity Assay Conditions: 3 μM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in 25 mM Tris-HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 minutes at 37°C followed by developing for 15 minutes at room temperature. |
| 供应商 | Active Motif |
| 背景 | HDAC6 (Histone Deacetylase 6) is a member of the class IIb mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulatedprotein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC6 is a unique enzyme that harbors a full duplication of its deacetylase homology region, which appears to contribute independently to the overall activity of HDAC6 protein. Alpha-tubulin and HSP90 are two known substrates of HDAC6, playing an important role in cellular mechanisms related to the microtubule network and HSP90-dependent events. HDAC6 also participates in regulating expression of a group of genes involved in the remodeling of chromatin during cell differentiation. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant HDAC6 (597-728, deleted mutant) is supplied at a concentration of 0.3 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. |
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Recombinant HDAC6 (597-728, deleted mutant) protein gel. HDAC6 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant HDAC6 (597-728, deleted mutant) protein activity assay. 3 μM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in reaction buffer for 30 minutes at 37°C followed by developing for 15 minutes at room temperature. |
