| 货号 | 31505 |
| 别名 | Histone Deacteylase 2, HD2, RPD3, YAF1 |
| 使用方法 | Recombinant HDAC2 is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active Motif offers a variety of protein screening tools including HDAC assay kits. HDAC Activity Assay Conditions: 3 μM Histone H3K9ac (1-21aa) peptide was incubated with HDAC2 protein in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 min at 37°C. HTRF assay was used for activity detection. |
| 供应商 | Active Motif |
| 背景 | HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC2 is associated with many different proteins as YY1 (a mammalian zinc-finger transcription factor). HDAC2 also forms transcriptional repressor complexes containing, among others, HDAC1 or RBBP4. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant HDAC2 protein was expressed in Sf9 cells and supplied at a concentration of 0.3 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. |
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Recombinant HDAC2 protein gel. HDAC2 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant HDAC2 activity assay. 3 μM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC2 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection. |
