| 货号 | 31504 |
| 别名 | Histone Deacetylase 1, HD1, RPD3, GON-10, RPD3L1 |
| 使用方法 | Recombinant HDAC1 is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active Motif offers a variety of protein screening tools including HDAC assay kits. HDAC Activity Assay Conditions: 3 μM Histone H3K9ac (1-21aa) peptide was incubated with HDAC1 protein in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM MgCl2, 1 mM KCl and 0.1 mg/ml BSA for 30 min at 37°C. HTRF assay was used for activity detection. |
| 供应商 | Active Motif |
| 背景 | HDAC1 (Histone Deacetylase 1) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC1 and HDAC2 are recruited to Mad-Max complexes, which associate with the Sin3 scaffold protein, and are required for the transcriptional repression of Mad-Max target genes. HDAC1 is also involved in the regulation of p53. HDAC1 is expressed in various tissues. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant HDAC1 protein was expressed in Sf9 cells and supplied at a concentration of 0.35 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP, 0.2 mg/ml 3× Flag peptide. |
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Recombinant HDAC1 protein gel. HDAC1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant HDAC1 activity assay. 3 μM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC1 protein in reaction buffer for 30 min at 37°C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection. |
