| 货号 | 31485 |
| 别名 | Lysine Demethylase 2A, Jumonji C Domain-Containing Histone Demethylase 1A, JHDM1A |
| 使用方法 | Recombinant KDM2A / FBXL11 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36me2 demethylase. Catalytic Ability: 13 turnovers/ enzyme molecule. Histone Demethylase Assay Conditions: 3.3 μM H3K36me2 peptide was incubated with 100 nM recombinant FBXL11 / KDM2A protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | Lysine (K)-specific demethylase 2A (KDM2A), also known as FBXL11, JHDM1A, CXXC8, nucleates at CpG islands and specifically demethylates ‘Lys-36’ of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 ‘Lys-36’ residue while it has weak or no activity for mono- and tri-methylated H3 ‘Lys-36’. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant FBXL11 / KDM2A protein was expressed in Sf9 cells at a concentration of 0.4 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.2 mg/ml 3×Flag peptide. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant KDM2A / FBXL11 protein gel. KDM2A / FBXL11 protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant KDM2A / FBXL11 protein activity assay. 3.3 μM H3K36me2 peptide was incubated with 100 nM FBXL11 / KDM2A in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 μM H3K36me2 peptide was used as negative control. |
