| 货号 | 31482 |
| 别名 | SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A |
| 使用方法 | Recombinant SMARCA4 / BRG1 (1448-1569), GST-tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA4 contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA4 is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Gene mutation causes Rhabdoid Tumor Predisposition Syndrome Type 2. SMARCA4 functions as a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It also interacts with glucocorticoid receptor (GR), TOPBP1 and progesterone receptor (PR) and is a component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Somatic mutations of SMARCA4 have been detected in some cancer cell lines and loss of SMARCA4 is associated with decreased survival in cancer patients. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 100 µg of recombinant SMARCA4 / BRG1 (1448-1569), GST-tag protein expressed in E. coli at a concentration of 3 mg/ml in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant SMARCA4 / BRG1 (1448-1569), GST-tag protein gel. SMARCA4 / BRG1 (1448-1569), GST-tag protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with SMARCA4 / BRG1 (1448-1569) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-GST antibody was used to detect reaction products. |
