| 货号 | 31478 |
| 别名 | KMT2C, HALR |
| 使用方法 | Recombinant MLL3 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: Histone methyltransferase with histone H3 lysine 4 methyltransferase activity. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 10 nM MLL3 Complex, 3.3 μM H3 (1-21) peptide at 2 hours at room temperature. |
| 供应商 | Active Motif |
| 背景 | TheMLL3 Complex (MLL3/WDR5/ASH2L/RBBP5/DPY30), also known as the MLL3 WARD Complex is a histone methyltransferase that, like MLLs 1, 2 and 4, preferentially methylates the activating histone mark lysine 4 of histone H3 (H3K4). The MLL3 (also known as KTM2C) subunit contains the catalytic SET domain that is responsible for substrate recognition and enzymatic activity, along with the WDR5 and RBBP5 interaction motifs necessary for assembly of the WARD complex. The MLL3 WARD complex forms part of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. The chromatin remodeling complex SWI/SNF also interacts with ASCOM through binding of the MLL3 (or MLL4) SET domain with the INI1 subunit of SWI/SNF. Knockdown of MLLs 3 and 4 expression results in a reduction of HOXC10, an estrogen-induced protein that is overexpressed in breast cancer. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant MLL3 Complex expressed in E. coli cells at a concentration of 0.25 mg/ml in 25 mM Tris, pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant MLL3 Complex protein gel. Recombinant MLL3 Complex run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant MLL3 Complex activity using AlphaLISA. MLL3 Complex was used in an AlphaLISA assay to determine enzyme linearity. This data was generated and kindly provided courtesy of ChemPartner. |
