| 货号 | 31477 |
| 别名 | NSD3; pp14328 |
| 使用方法 | Recombinant WHSC1L1 / NSD3 - SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 400 nM WHSC1L1 / NSD3 - SET at 2 hours at room temperature. AlphaLISA was used for detection. |
| 供应商 | Active Motif |
| 背景 | WHSC1L1 (Wolf-Hirschhorn Syndrome Candidtae 1-Like 1) is a histone methyltransferase of NSD (nuclear receptor binding SET domain protein ) family. WHSC1L1 can recognize and methylate several lysine marks if using histone as substrate. However, if using nucleosome as substrate, NSD3 preferentially methylates lysine 36 of histone H3. The WHSC1L1 gene is amplified in several cancers, including lung cancer and head and neck cancer, and may play a role in carcinogenesis. WHSC1L1 / NSD3 - SET includes the SET domain of WHSC1L1. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in Drosophila proteins Su(var)3-9, EZH2, and TrxG, and plays a crucial role in substrate recognition and enzymatic activity. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant WHSC1L1 / NSD3 - SET peptide (aa 1021-1320) containing an N-terminal GST tag expressed in E. coli cells at a concentration of 0.8 mg/ml in 25 mM Tris, pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant WHSC1L1 / NSD3 - SET protein gel. Recombinant WHSC1L1 / NSD3 - SET run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant WHSC1L1 / NSD3 - SET activity using AlphaLISA. WHSC1L1 / NSD3 - SET was used in an AlphaLISA assay to determine enzyme linearity. This data was generated and kindly provided courtesy of ChemPartner. |
