| 货号 | 31475 |
| 别名 | STO; KMT3B; SOTOS; ARA267; SOTOS1 |
| 使用方法 | Recombinant NSD1-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: H3K36 and H4K20 methyltransferase HMT Assay Conditions: 30 ng/μl recombinant nucleosomes were incubated with 30 ng/μl recombinant NSD1-SET protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | NSD1 (Nuclear Receptor Binding SET Domain Protein 1) is a histone methyltransferase that preferentially methylates lysine 36 of histone H3 and lysine 20 of histone H4 in vitro. NSD1 is a transcriptional intermediary factor capable of both negatively or positively influencing transcription, depending on the cellular context. NSD1 protein contains a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor-associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor as well as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su(var), Enhancer of zeste (EZH2), and Trithorax Domain Protein 1 on chromosome 5 and Nucleoporin, 98 kd on chromosome 11.NSD1-SET includes the SET domain of NSD1. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | 50 µg of recombinant NSD1-SET peptide (aa 1852-2082) containing an N-terminal GST tag expressed in E. coli cells at a concentration of 1.0 mg/ml in 25 mM Tris, pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant NSD1-SET protein gel. Recombinant NSD1-SET run on an SDS-PAGE gel and stained with Coomassie blue. | |
NSD1-SET activity assay using Recombinant Nucleosomes as substrates. Recombinant Nucleosomes were used as substrates in an assay measuring the methyltransferase activity of NSD1-SET. Activity was detected by fluorography. |
