| 货号 | 31474-AM |
| 别名 | DOT1, KMT4 |
| 使用方法 | Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: H3K79 methyltransferase HMT Assay Conditions: 30 ng/μl recombinant nucleosomes were incubated with 30 ng/μl recombinant DOT1L protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | DOT1L(Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the DrosophilaGrappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant DOT1L (1-416), active protein expressed in E. coli cells at a concentration of 0.4 mg/ml in 25 mM Tris, pH 7.4, 150 mM NaCl and 5% glycerol. |
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Recombinant DOT1L (1-416), active protein gel. Recombinant DOT1L (1-416), active run on an SDS-PAGE gel and stained with Coomassie blue. | |
DOT1L (1-416), active activity assay using Recombinant Nucleosomes as substrates. Recombinant Nucleosomes were used as substrates in an assay measuring the methyltransferase activity of DOT1L (1-416), active. Activity was detected by fluorography. |
