| 货号 | 31460-AM |
| 别名 | KABUK2; bA386N14.2 |
| 使用方法 | Recombinant UTX / KDM6A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K27me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant UTX / KDM6A protein, and 3.3 μM H3K27me3 peptide at 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | KDM6A (lysine (K)-specific demethylase 6A), also known as UTX (Ubiquitously Transcribed Tetratricopeptide Repeat, X Chromosome) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 27 residues of histone H3, while it has no activity on monomethylated H3K27 residues. KDM6A plays a central role in posterior development by regulating HOX gene expression. Demethylation of lysine 27 is concomitant with methylation of lysine 4 of histone H3 and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant UTX / KDM6A protein expressed in Sf9 cells at a concentration of 0.6 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant UTX / KDM6A protein gel. UTX / KDM6A protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
UTX / KDM6A activity assay. Recombinant UTX / KDM6A activity measured using a demethylation assay. MALDI-TOF was used for detection. | |
UTX / KDM6A AlphaLISA assay. UTX / KDM6A enzyme was used in an AlphaLISA assay to determine enzyme linearity, 2OG Km and IC50 of two reference compounds. 10 nM-20 nM KDM6A was used to develop an HTS screening assay and well-to-well reproducibility was also assessed at Z’=0.64. Demethylated peptide (H3K27me2) was measured using methylation-specific antibody. This data was generated and kindly provided courtesy of ChemPartner. |
