| 货号 | 31459 |
| 别名 | Lysine Demethylase 4D, Jumonji Domain-Containing Protein 2D, JHDM3D |
| 使用方法 | Recombinant JMJD2D / KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2D / KDM4D protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant JMJD2D / KDM4D protein expressed in Sf9 cells at a concentration of 0.8 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol. |
| 参考文献 | This protein is described in the following references: Fodor BD et al.(2006)Genes Dev.20:1557-1562 Beyer S et al.(2008)J Biol Chem. 283:36542-36552. doi: 10.1074/jbc.M804578200 |
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Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
JMJD2D / KDM4D activity assay. Recombinant JMJD2D / KDM4D activity measured using a demethylation assay. MALDI-TOF was used for detection. | |
Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner. |
