| 货号 | 31458 |
| 别名 | Lysine Demethylase 4C, Jumonji Domain-Containing Protein 2C, JHDM3C |
| 使用方法 | Recombinant JMJD2C / KDM4C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 and H3K36me3 demethylase. Histone Demethylase Assay Conditions: 3.3 μM H3K9me3 (aa 1-21) peptide was incubated with different concentrations of recombinant JMJD2C / KDM4C protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | KDM4C (lysine (K)-specific demethylase 4C), also known as JMJD2C (Jumonji Domain Containing 2C) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4C has no activity for mono- and dimethylated H3K9 and H3K36. Chromosomal aberrations and overexpression of KDM4C are associated with esophageal squamous cell carcinoma. KDM4C regulates colonosphere formation in colorectal cancers by mediating the crosstalk between Wnt and Notch signaling pathways. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant JMJD2C / KDM4C protein was expressed in Sf9 cells at a concentration of 0.45 μg/μl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| 参考文献 | This protein is described in the following references: Whetstine JR et al.(2006)Cell125:467-481 |
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Recombinant JMJD2C / KDM4C protein gel. JMJD2C / KDM4C protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant JMJD2C / KDM4C activity assay. JMJD2C / KDM4C enzyme was used in an HTRF assay to determine enzyme linearity. 3.3 μM H3K9me3 peptide was incubated with JMJD2C / KDM4C in reaction buffer for 1 hour at room temperature. Demethylated peptide (H3K9me2) was measured using H3K9me2-specific antibody. |
