| 货号 | 31457 |
| 别名 | Lysine Demethylase 4A, Jumonji C Domain-Containing Histone Demethylase 3A, JHDM3A |
| 使用方法 | Recombinant JMJD2A / KDM4A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 and H3K36me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2A / KDM4A protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4A has no activity for mono- and dimethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant JMJD2A / KDM4A protein expressed in Sf9 cells at a concentration of 0.82 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. |
| 参考文献 | This protein is described in the following references: Zhang D et al.(2005)Mol Cell Biol.25:6404-6414 Gray SG et al.(2005)J Biol Chem.280:28507-28518 |
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Recombinant JMJD2A / KDM4A protein gel. JMJD2A / KDM4A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
JMJD2A / KDM4A activity assay. Recombinant JMJD2A / KDM4A activity measured using a demethylation assay. MALDI-TOF was used for detection. |
