| 货号 | 31455 |
| 别名 | CXXC2; Fbl10; PCCX2; JHDM1B |
| 使用方法 | Recombinant FBXL10 / KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36me2 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 300 nM Recombinant FBXL10 / KDM2B protein, and 3.3 μM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant FBXL10 / KDM2B protein expressed in Sf9 cells at a concentration of 0.8 mg/ml in 25 mM HEPES, pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant FBXL10 / KDM2B protein gel. FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
FBXL10 / KDM2B activity assay. Recombinant FBXL10 / KDM2B activity measured using a demethylation assay. MALDI-TOF was used for detection. |
