| 货号 | 31450-AM |
| 别名 | CT9, BRD6 |
| 使用方法 | Recombinant BRDT (21-137) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | BRDT is similar to the RING3 protein family. It possesses two bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant BRDT (21-137) protein expressed in E. coli at a concentration of 1.9 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant BRDT (21-137) protein gel. BRDT (21-137) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant BRDT (21-137) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRDT (21-137) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products. |
