| 货号 | 31447 |
| 别名 | FAC1; NURF301 |
| 使用方法 | Recombinant BPTF / FALZ (2791-2911) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | BPTF (Bromodomain PHD finger transcription factor) is a histone-binding component of NuRF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. BPTF protein contains a DNA-binding domain, a zinc finger motif and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. BPTF also specifically recognizes H3 tails trimethylated on lysine 4 (H3K4me3), which mark transcription start sites of virtually all active genes. It may also regulate transcription through direct binding to DNA or transcription factors. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant FALZ (2791-2911) protein expressed in E. coli cells at a concentration of 2 μg/μl in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. |
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Recombinant BPTF / FALZ (2791-2911) protein gel. BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant BPTF / FALZ (2791-2911) activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products. |
