| 货号 | 31444 |
| 别名 | WSTF; WBSCR9; WBSCR10 |
| 使用方法 | Recombinant BAZ1B (1340-1457) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | Bromodomain adjacent to zinc finger domain, 1B (BAZ1B) is a member of the bromodomain protein family. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BAZ1B is an atypical tyrosine protein kinase that plays a central role in chromatin remodeling and acts as a transcriptional regulator. It is an essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes and has a role in RNA polymerase I and RNA polymerase III transcription. In the complex, BAZ1B mediates the recruitment of the WICH complex to replication foci during DNA replication. BAZ1B and the WICH complex are also involved in DNA damage response through phosphorylation of Tyr142 of histone H2AX (H2AXY142ph), a mark that distinguishes between apoptotic and repair responses to genotoxic stress. BAZ1B is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, BAZ1B plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Deletion of BAZ1B leads to Williams-Beuren syndrome. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant BAZ1B (1340-1457) protein expressed in E. coli at a concentration of 4.7 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant BAZ1B (1340-1457) protein gel. BAZ1B (1340-1457) protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant BAZ1B (1340-1457) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BAZ1B (1340-1457) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products. |
