| 货号 | 31440-AM |
| 别名 | N143; WDR9; C21orf107 |
| 使用方法 | Recombinant BRWD1 (1310-1430) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | BRWD1 (Bromodomain and WD repeat domain containing 1) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. In mouse, this BRWD1 is a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain. This domain associates with a component of the SWI/SNF chromatin remodeling complex and may function to regulate chromatin remodeling. BRWD1 plays a role in the regulation of cell morphology and cytoskeletal organization and is also required in the control of cell shape. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant BRWD1 (1310-1430) protein was expressed in E. coli cells at a concentration of 1.6 μg/μl in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. |
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Recombinant BRWD1 (1310-1430) protein gel. BRWD1 (1310-1430) protein was run on a 12% SDS-PAGE gel and stained with Coomassie blue. | |
HTRF assay for BRWD1 (1310-1430) activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products. |
