| 货号 | 31435 |
| 别名 | JHDM1F; MRXSSD; ZNF422 |
| 使用方法 | Recombinant PHF8 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 1 µM H3K9me1 or H3K4me3K9me1 peptide was incubated with different concentrations of recombinant PHF8 protein in reaction buffer containing 50 mM HEPES-NaOH pH 7.5, 50 µM 2OG, 50 µM Ascorbate, 25 µM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hour at room temperature. HTRF reaction was used for detection. This product has been used in the following publication(s): Ulicna, et. al. Biochim Biophys Acta. 2018 Mar;1863(3):266-275. |
| 供应商 | Active Motif |
| 背景 | PHF8(PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B(KDM7B) and JmjC domain-containing histone demethylation protein 1D-B(JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1), Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). PHF8 depletion in mammalian neuronal cells results in decreased expression of follistatin and increased methylation of H3K9 and H3K27. It has also been suggested to function in gene silencing during brain development. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant PHF8 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration. |
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