| 货号 | 31433 |
| 别名 | Lysine Demethylase 5C, Jumonji, AT Rich Interactive Domain 1C (RBP2-Like) |
| 使用方法 | Recombinant JARID1C / KDM5C is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 100 nM Recombinant JARID1C / KDM5C protein, and 3.3 μM H3K4me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection. |
| 供应商 | Active Motif |
| 背景 | Lysine (K)-specific demethylase 5C (KDM5C), also known as Jumonji, AT rich interactive domain 1C (JARID1C), is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. It is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5C participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and CoREST pAb at neuron-restrictive silencer elements. Mutations in KDM5C have been associated with X-linked mental retardation. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant JARID1C / KDM5C protein expressed in Sf9 at a concentration of 0.85 mg/ml in 40 mM HEPES pH 7.4, 150 mM NaCl and 20% glycerol. |
| 参考文献 | This protein is described in the following references: Xu J et al.(2008)PLoS One 3:e2553. doi: 10.1371/journal.pone.0002553 Iwase S et al.(2007)Cell128:1077-1088 |
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Recombinant JARID1C / KDM5C protein gel. JARID1C / KDM5C protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
JARID1C / KDM5C activity assay. Recombinant JARID1C / KDM5C activity measured using a demethylation assay. MALDI-TOF was used for detection. |
