| 货号 | 31432 |
| 别名 | Lysine Demethylase 5B, Cancer/Testis Antigen 31, Retinoblastoma-Binding Protein 2 Homolog 1, Histone Demethylase JARID1B |
| 使用方法 | Recombinant JARID1B / KDM5B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K4me3 demethylase. Catalytic Rate: 20 turnovers/ enzyme molecule Histone Demethylase Assay Conditions: 3.3 μM H3K4me3 (aa 1-21) peptide was incubated with different concentrations of recombinant JARID1B / KDM5B protein in reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection. |
| 供应商 | Active Motif |
| 背景 | Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant JARID1B / KDM5B protein expressed in Sf9 at a concentration of 0.2 mg/ml in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 3X FLAG peptide. |
| 参考文献 | This protein is described in the following references: Yamane K et al.(2007)Mol Cell25:801-812 Barrett A et al.(2007)Int J Cancer121:265-275 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant JARID1B / KDM5B protein gel. JARID1B / KDM5B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant JARID1B / KDM5B protein activity assay. 3.3 μM H3K4me3 peptide was incubated with 50 nM JARID1B / KDM5B in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 μM H3K4me3 peptide was used as negative control. Catalytic Ability: 20 turnovers/ enzyme molecule | |
Recombinant JARID1B / KDM5B protein activity assay. 3.3 μM H3K4me3 peptide was incubated with JARID1B / KDM5B in reaction buffer for 1 hours at room temperature. JARID1B / KDM5B enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me2) was measured using H3K4me2-specific antibody. |
