| 货号 | 31411 |
| 别名 | ANM1, HCP1, IR1B4, HRMT1L2 |
| 使用方法 | Recombinant PRMT1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.Specific Activity: H3R8 and H4R3 methylase. HMT Assay Conditions: 30 ng/μl recombinant octamers were incubated with 30 ng/μl recombinant PRMT1 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography. |
| 供应商 | Active Motif |
| 背景 | PRMT1(protein arginine N-methyltransferase 1) is a member of the protein arginine N-methyltransferase (PRMT) family capable of monomethylating and asymmetrically dimethylating arginine residue in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. PRMT1 specifically methylates arginine 3 on histone H4 (H4R3), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, PRMT1 represses STAT1 transcriptional activity in the late phase of interferon gamma (IFN-gamma) signaling. PRMT1 may be involved in the regulation of TAF15 transcriptional activity and may act as an activator of estrogen receptor (ER)-mediated transactivation. It may also play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation by modulating p38 MAPK pathways. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant PRMT1 protein expressed in Sf9 cells at a concentration of 0.2 mg/ml in 25 mM HEPES pH 7.5, 300 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol. |
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Recombinant PRMT1 protein gel. PRMT1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
PRMT1 activity assay. HMT assay measuring the activity of Recombinant PRMT1. Activity was detected by fluorography. |
