Recombinant EHMT2 (G9a) protein
货号：
31410 基本售价：
5872.5 元 规格：
20 μg
产品信息
概述| 货号 | 31410 |
| 别名 | EHMT2; BAT8; GAT8; NG36; KMT1C; C6orf30 |
| 使用方法 | Recombinant G9a is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. |
性能| 供应商 | Active Motif |
| 背景 | G9a, also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. G9a also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Full length recombinant G9a protein expressed in Sf9 cells at a concentration of 1.5 mg/ml in 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 20% glycerol. |
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参考图片Recombinant G9a protein gel.
G9a protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. |
