| 货号 | 31385 |
| 别名 | BRG1-associated factor 180 (BAF180), Polybromo 1, PB1; BAF180 |
| 使用方法 | Recombinant PBRM1 (613-734) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant PBRM1 (613-734) protein expressed in E. coli at a concentration of 2.98 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant PBRM1 (613-734) protein gel. PBRM1 (613-734) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant PBRM1 (613-734) HTRF activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with PBRM1 (613-734) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-Flag antibody was used to detect reaction products. |
