| 货号 | 31381 |
| 别名 | BP75; NAG4; CELTIX1 |
| 供应商 | Active Motif |
| 背景 | Bromodomain-containing protein 7 (BRD7) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD7 interacts with several proteins, including DVL1, PTPN13, IRF2 and HNRPUL1 and functions in the regulation of transcriptional activation and chromatin remodeling. Specifically, BRD7 has been shown to bind dishevelled-1 (DVL1) and enhance Wnt signaling via inhibition of GSK3β. BRD7 also associates with histones and E1B-AP5. In particular, it binds acetylated histone peptides, most notably H3 peptide acetylated at Lys14. BRD7 also inhibits G1-S progression by transcriptional regulation of molecules in the Ras and Rb pathways. BRD7 also suppresses tumorigenicity through binding and acetylation of p53 that results in efficient recruitment of p53 to target promoters and subsequent oncogene-induced senescence. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant BRD7 (129-236) protein expressed in E. coli at a concentration of 5 mg/ml in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant BRD7 (129-236) protein gel. BRD7 (129-236) protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant BRD7 (129-236) activity using AlphaScreen. BRD7 (129-236) titration was assessed using an AlphaScreen® assay. Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner. |
