| 货号 | 31376-AM |
| 别名 | ANCCA; CT137; PRO2000 |
| 使用方法 | Recombinant ATAD2 (981-1108) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | ATPase family AAA domain-containing 2 (ATAD2) protein belongs to the AAA protein family which includes a diverse set of proteins with the capacity to couple ATPase-derived chemical energy to mechanical energy resulting in remodeling or translocation of the targeted macromolecular substrate. These proteins are involved in a number of cellular processes ranging from protein degradation and DNA replication to membrane fusion and the movement of microtubule motors. ATAD2 is thought to act as a transcriptional coactivator of the nuclear receptor ESR1 that is required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. Furthermore, ATAD2 may also play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. In addition, there is evidence that ATAD2 may be required for histone hyperacetylation. Human ATAD2 consists of two AAA+ domains and a bromodomain. Bromodomains recognize acetylated histone lysine residues and function as ‘readers’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. The ATAD2 bromodomain specifically interacts with H4K5ac and may contribute to cancer cell proliferation since it has been associated with poor prognosis in breast and testicular cancer. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant ATAD2 (981-1108) protein expressed in E. coli at a concentration of 3 mg/ml in 25 mM Tris pH 7.4, 150 mM NaCl and 5% glycerol. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Recombinant ATAD2 (981-1108) protein gel. ATAD2 (981-1108) protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant ATAD2 (981-1108) activity using AlphaScreen. ATAD2 (981-1108) titration was assessed using an AlphaScreen® assay . Titration curves were generated to show signal response in the presence of modified peptide substrate at increasing protein concentrations. This data was generated and kindly provided courtesy of ChemPartner. |
