| 货号 | 31372 |
| 别名 | EP300; KAT3B; RSTS2 |
| 使用方法 | Recombinant p300 (1041-1161) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | E1A binding protein p300 (EP300)orp300 regulates cellular growth and differentiation and is also important in preventing tumor growth. Its interaction with adenovirus E1A protein is thought regulate the transforming capacity of E1A. p300 is a transcriptional coactivator with histone acetyl transferase (HAT) activity. It can acetylate all four core histones and regulates transcription via chromatin remodeling. p300 also functions as an acetyltransferase for non-histone targets. Specifically, p300 acetylates Lys131 of ALX1 and acts as its coactivator in the presence of CREBBP. p300 is also thought to indirectly increase the transcriptional activity of p53 through acetylation of SIRT2 and subsequent attenuation of its deacetylase function. Additionally, HDAC1 acetylation by p300 leads to HDAC1 inactivation. p300 also acts as a TFAP2A-mediated transcriptional coactivator in the presence of CITED2 and as a coactivator of NEUROD1-dependent transcription of Secretin and p21. Additionally, p300 binds to phosphorylated CREB and mediates cAMP gene regulation. It also regulates terminal differentiation of intestinal epithelial cells. In the case of HIV-1 infection, p300 is recruited by the viral protein TAT and regulates TATs transactivating activity, and may aid induction of chromatin remodeling of proviral genes. p300 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant p300 (1041-1161) protein was expressed in E. coli cells at a concentration of 1.5 μg/μl in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. |
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Recombinant p300 (1041-1161) protein gel. p300 (1041-1161) protein was run on a 15% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant p300 (1041-1161) activity assay. 3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with p300 (1041-1161) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products. |
