| 货号 | 31370 |
| 别名 | K (lysine) acetyltransferase 2B (KAT2B), P300/CBP-associated factor, P/CAF |
| 使用方法 | Recombinant PCAF (715-829) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. |
| 供应商 | Active Motif |
| 背景 | P300/CBP-associated factor (PCAF), also known as K (lysine) acetyltransferase 2B (KAT2B), is a transcriptional coactivator associated with p53 that contains acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. PCAF competes with E1A for binding to p300/CBP, which associate with various sequence-specific factors involved in cell growth and/or differentiation, including c-Jun and the adenoviral oncoprotein E1A. PCAF exerts histone acetyltransferase activity in association with core histones (H3 and H4) and with nucleosome core particles, and functions to promote transcriptional activation. PCAF has also been shown to inhibit cell cycle progression and to counteract the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, PCAF is recruited by the viral protein TAT to regulate TAT’s transactivating activity and may help induce chromatin remodeling of proviral genes. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant PCAF (715-829) protein expressed in E. coli at a concentration of 3 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol. |
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Recombinant PCAF (715-829) protein gel. PCAF (715-829) protein was run on an SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant PCAF (715-829) activity using AlphaScreen. PCAF (715-829) activity was assessed using an AlphaScreen® assay testing various buffer formulations. This data was generated and kindly provided courtesy of ChemPartner. |
