| 货号 | 31421 |
| 别名 | hCG_40738 |
| 使用方法 | Recombinant TET3 (824-1795) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling. TET Activity Assay Conditions: 250 nM of 30bp DNA oligos (sequence: 5-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3, contains a 5mCpG site) was incubated with 100 nM recombinant TET3 (824-1795) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37C for 1 hr. Activity was detected by Dot-blot. |
| 供应商 | Active Motif |
| 背景 | TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation. TET3 plays an important role in DNA methylation reprogramming processes in the mammalian zygote. TET3 may also contribute to somatic cell nuclear reprogramming during animal cloning. Both of CXXC domain and catalytic domain are important for TET3 regulating key genes of Xenopus eye and neural development. |
| 运输条件 | Dry Ice |
| 存放说明 | Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 外观 | Recombinant TET3 (824-1795) protein is supplied at a concentration of 0.1 µg/µl in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP and 0.2 mg/ml 3× Flag peptide. |
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Recombinant TET3 (824-1795) protein gel. Recombinant TET3 (824-1795) protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. | |
Recombinant TET3 (824-1795) protein activity assay Recombinant TET3 (824-1795) protein activity was measured using TET activity assay and detected by Dot-blot. |
